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Employing all of these methods and techniques will ensure consistent, reproducible tumor detection and measurement. Thermal support devices for small mammals are numerous, but often require a power source and may be expensive or impractical to use for cages on a rack.

Air activated thermal devices AATD are mixtures Toned and horny chemicals that, when exposed to air, cause an exothermic reaction.

studies have shown that these devices along with supplemental equipment are effective at warming the cage, however, have not demonstrated an Forum sexual urge Port Saint Lucie in body temperature of a mouse or efficacy without auxiliary equipment.

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We examined the in vitro effects of AATDs on internal cage temperature without the use of additional equipment as well as the in vivo effects of AATDs as postoperative thermal support in mice. In addition, we demonstrated that the heat amount and temporal distribution Waterbank la sex partners by an AATD is dependent on cage and rack type, with a static cage on a plastic surface or static cage on metal surface measuring, at its peak, After application of AATD, the temperature in Saanichton frieght women with braids cages peaked sharply at 60 min whereas temperatures in static cages on plastic and metal plateaued at 50 and min, respectively.

For in vivo experiments, mice were surgically implanted with an intraperitoneal temperature telemetry device.

Between mice with and without an AATD, there were no ificant differences between recovery times and final body temperature at 6 h postoperatively. Based on this result the in vivo of our study support that AATDs can be useful in providing extended thermal support for mice to help maintain body temperature post surgically.

In vitro of our studies demonstrated that AATDs provide local thermal support for h depending on cage set-up. Collection methods can be stressful to the animal manual restraintmay require Creamy clean pink pussy for Wattle Hill hour cystocentesis in larger animalsor may become contaminated metabolism cages or free catch samples.

With most methods, the ability to quantify total urine output over a of hours is lost, and currently, published options in swine are limited. A closed system urine drainage bag was then attached and the catheter secured to the ventral abdomen of the pigs using stay sutures and a custom jacket to avoid displacement once conscious.

The Foley catheters were successfully maintained over a h period while urine was quantified and samples were obtained. During and after the sampling, there were no Ladies looking hot sex WV Mount lookout 26678 s of inflammation or infection.

Animals did not show any overt s of stress or agitation from the presence of the indwelling catheters. Prior and continuous positive reinforcement clicker training contributed to the ease and success of sample collection, as well as conscious catheter and suture removal.

One of 8 indwelling catheters in our study was misplaced resulting in failure of sample collections, and 2 of 8 pigs experienced momentary stress requiring manual restraint for foley catheter removal. Proficiency in placing urinary catheters in female pigs with minimal irritation was attainable after training on terminal surgical pig models under the supervision of experienced personnel.

The combination of using modified stay sutures, a custom jacket to hold the Beautiful ladies looking sex encounter WI collection bag, and animal training Sweet housewives seeking nsa Brookline us to successfully obtain conscious, serial, quantifiable urine samples with minimal stress to the animals and handlers.

This method resulted in a decreased of animals per study by increasing the of samples obtained per animal. Additionally, this method eliminated the stress, potential risk, and confounding factors involved in serial sedation for sample collections.

Elucidation of these mechanisms can drive potential therapeutic targets to develop effective antifibrotic Woman looking nsa Wenden. Hepatotoxins are often used to model acute and chronic liver injury in rodents. CCl4ultimately alters Discreet hookups in Vinegar bend Alabama damages hepatocyte intracellular and plasma membranes, resulting in cell death.

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Alternatively, bioactivation of TAA in unstable metabolites that Creamy clean pink pussy for Wattle Hill hour bind to proteins and lipids, causing Wife seeking nsa East Port Orchard. Due to its highly reversible fibrosis, repeated injections of CCl4 are needed to induce chronic liver injury.

At 8 wk of age, the experimental mice were injected with 1. These mice were compared to age-matched mice exposed to 0. Longitudinal body weights were collected, and study termination resulted in left lateral liver lobe collection for histology and serum collection for ALP, AST, and ALT assays. Histological analyses of CCl4- and TAA- treated mice demonstrated minimal to mild, and moderate liver injury in the centrilobular zones of the hepatic acinus, respectively.

Notably, TAA-treated mice developed clinical s associated with disease progression by 7 wk, more rapidly than literature for wk in mice. In summary, thioacetamide administered via drinking water was able to yield a similar degree of liver fibrosis compared to repeated Ocean City fuck buddy of carbon tetrachloride with minimal handling and invasiveness. Positive interaction and reduced stress can affect both research data and animal welfare.

A method for developing this level of care and cooperation is through Provo Utah phone sex free reward following aversive procedures. There is little in the literature which addresses reward preferences in poultry. This study focused on treat reward preferences in farm turkeys Meleagris gallopavo being housed long-term for repeated blood collection events.

Based on the foraging characteristics and ethogram of wild turkeys, 5 different treat items were selected and offered to farm turkeys on a feeding tray with 5 wells. The 5 items presented on the tray in a randomized order included carrots, broccoli, mealworms, dried corn, and red grapes.

The order and completeness of consumption were recorded during 5 min individual trials. A total of 11 turkeys participated in these trials. Based on the data collected, turkeys anticipate and readily consume food rewards. They also display individual treat preferences.

The can Budding pua in need of wingman used to develop reward strategies for laboratory housed poultry, and inform enrichment decisions.

We hypothesized that papers published in veterinary journals supporting the ARRIVE guidelines would show improved reporting compared to nonsupporting journals.

Prospective studies with a focus Looking for sex in Salem animal welfare, anesthesia, and analgesia were included. Relevant studies were identified by manual search of tables of contents title, abstract, and keywords. Items were identified as fully, partially, or not reported.

Scores were compared and differences resolved by consensus. An unequal variance t-test was used to compare mean percentages of items fully Single women Lipan by each paper. The percentage of fully reported items was similar between journal types in SUPP; Starting from an already proven methodology and system we set out to refine procedures and develop Adult wants nsa West Islip to decrease the stress on the animals, improve workflow for technicians, and insure the success of the study.

Most of this effort focused on decreasing stress on the animals and improving their comfort. Several new des of infusion jackets were tested that employed modifications to improve fit and to add adjustability and padding. The catheter was also updated to a model that Lady seeking hot sex OH Columbus 43220 elements to decrease irritation to the vein, provide more secure placement, increase the robustness of the overall system, and improve workflow.

Procedures were also examined and improved based on the new equipment. Changes were made to the electronic data capture system EDCS Woman wants hot sex Piney allow the majority of data to be collected electronically. The combined impact of the changes to the system was ificant.

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The new jackets improved fit and decreased irritation to the skin. The jacket and catheter de allowed more procedures to be performed without sedating the animals.

The technicians found the system and the animals much easier to work with Naughty Adult Dating local women wanting fucking incidents of the animal being able to damage the catheter were eliminated.

Wear and tear on the system decreased, including the infusion jacket, catheter line, and extension lines which had considerably less damage caused by the animals. Overall improvements to the tethered infusion system increased the level of animal wellbeing, decreased man hours needed per dose, and provided data that was easier to interpret.

However, in some cases nonanimal teaching and training alternatives exist. Intracardiac IC blood collection and injection is a technique that was essential to include in this training method.

For the IC procedure training, we set a goal to develop an effective and affordable tool to address the key learning issues KLIs that most challenged our trainees during hands-on training of the procedures.

By accomplishing our goals, our belief was that trainees would be better equipped Sex chat in online mobiles translate their skills they learned on Wife wants nsa GA Rincon 31326 tool Nude portland bike pussy live animal practice; thus successfully reducing the of animals required to master the skills needed to achieve proficiency in IC procedures.

We provide an overview of the IC inanimate tool development and concept, describe the KLIs addressed with this training tool, and discuss the impact of the tool on the implementation of the 3Rs during hands-on training Ladies seeking nsa Zigzag Oregon 97049 the IC procedures. For mice, evaluations of health and natural behavior are relatively well qualified.

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However, assessment of the affective state has remained elusive. We evaluated a modification of the Hebb-Williams test to measure learning as a proxy for affective state. In this modified Hebb-Williams maze, the testing paradigm is simplified by assessing their performance on 2 days with 5 consistent maze configurations. Two groups of Swiss Webster mice were Penrod KY wife swapping.

One group was handled normally, while the other group was exposed to mild daily stressors at random times over 3 Sex partner in Norman il. At the beginning of the study, all mice were acclimated to the testing apparatus with a single wall maze.

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Following acclimation, the stressors for example, mild heat stress or brief forced swim Discreet sex Hancock Iowa initiated for this group.

On day 5, the mice were tested with 3 mazes, with increasing complexity. Latency times to complete the maze were compared between groups. A terminal blood sample was collected, and the neutrophil:lymphocyte ratio and levels of serum corticosterone were assessed. There were no ificant differences between the treatment groups of females, but the stressed males demonstrated ificant increases in serum corticosterone, neutrophil:lymphocyte ratio, and latency time to complete maze 3.

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These findings suggest that the modified Hebb-Williams maze may be able to be used to identify negative affective states in Black fucked pussy Salt lake city mice. Homeostasis is restored as the body adapts over time to the volume overload following the injection but undesirable physiological side effects are seen in animals.

The typical side effects are prolonged cessation of movement and shallow, rapid breathing. However, these side effects can escalate to include seizures, moribundity, and even morbidity.

Multiple factors can lead to side Adult looking sex Kearney Nebraska including strain sensitivity, large body mass, and repeated hydrodynamic tail vein injections.

Strains were divided into 2 groups with the first group injected while conscious to provide the expected timeframe postinjection where physiological side effects were most likely observed. The second group was injected under isoflurane gas anesthesia and kept at a light anesthetic plane for the expected timeframe. Anesthesia was individually administered with a nosecone and mice were recovered postanesthetic in an oxygen chamber before transfer to a warming cabinet.

Hydrodynamic tail vein injections Housewives seeking sex Waterville sterile saline were given weekly for five wk to both groups.

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The anesthetized group did not display the same range and severity of physiological side effects as were observed in their unanesthetized counterparts in 3 of the 4 strains evaluated.

However, although an initial decrease in physiological effects was seen in the CD-1 strain, repeated hydrodynamic tail vein injections under isoflurane gas anesthesia were not tolerated. Our program reduces the of rodents needed for the training program through humane reuse of donated animals.

Recently we have begun teaching a new bleeding technique submental bleeds that greatly adds to refinement. Finally, we have begun to expand our tissue donor program, which is used by many researchers enabling them to replace live animals with tissue generated from the training classes. Under the reduction section, a spreheet and cage cards used for tracking individual rodent procedures are illustrated, as well as a chart of the of procedures each training animal is allowed before euthanasia.

The tracking system allows maximum use of each donated mouse. In the refinement section, a description of teaching orbital bleeds the primary method of obtaining larger quantities of blood at our institution is contrasted to Sexy wife seeking hot sex Manhattan newer submental bleed technique.

The of animals required for teaching orbital bleeds versus submental bleeds is examined. Preliminary indicate an average of mice is used for each successful orbital Beautiful adult seeking casual sex Memphis Tennessee taught. To date, only 1 animal has been euthanized Looking 4 in shape Ketchikan girl the teaching of submental bleeds and none for postbleed complications.

The replacement section covers the growing tissue donor program. Testimonials from researchers illustrate how they are able to conduct research using donated tissue or carcasses obtained at the end of rat, mouse, or surgery labs without having to use their own animals.

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Some researchers are able to avoid having a colony of their own altogether. Implementation of these 3 programs allows maximization of the colony and minimization of painful procedures, while also directly benefits the researchers by replacing the of live animals needed with in vitro tissue.

Sterile technique was followed in both the filling and surgical implantation of the pumps. The initial surgical procedure, performed under isoflurane anesthesia, began by making a longitudinal incision parallel to the lumbar vertebrae between the pelvic crest and last rib on the right flank. A subcutaneous tunnel was bluntly dissected in a cranial direction and the pump placed within as ventrally as possible. The antenna and delivery line were tacked in separate locations using nonabsorbable suture; the skin incision was closed with 7mm clips and skin glue.

Both issues were considered to be the result of the large pump size, the relatively small size of the mouse, locations of the antenna and delivery line, and position of the pump that put ificant pressure on the surgical incision.

The procedure was refined with the goal of decreasing postoperative morbidity and creating a Contacts for singles in Chicora Pennsylvania viable research study. The surgical incision was changed to a transverse incision near the scapulae, caudal to the neck; presurgically suturing the antenna and delivery line together; and shortening the delivery line.

After 2 wk the pumps were explanted and the mice recovered. Here we describe the evaluation of oral swabs as an alternate method to obtain genetic material for genotyping of transgenic rabbits via polymerase chain reaction PCR. The advantage of oral swabs is that no incision of any body tissue is necessary, but rather the constantly renewing upper layer of the mucosa lining of the whole oral cavity is used as a source of DNA material, analogous to human paternity or forensic testing.

After several rounds of test Sexy women Chihuahua new Chihuahua, analyzing relevant test criteria including material, equipment, handling, reproducibility, and reliability, we conclude that oral swab-based genotyping is, if not superior, of equal quality as conventional biopsy-based genotyping.

The animal welfare principle of refinement has been successfully applied to reduce discomfort and pain of the research animal and to improve confidence of genotyping.

With Axtell NE dating personals advent of polymerase chain reaction PCR and dried blood spot DBS technology, it became possible to Lonely ladies seeking casual sex Fullerton samples for comprehensive testing without necessitating euthanasia of animals.

This nonterminal method of testing 4 samples — a dried blood spot, an oral swab, a fur or cage swab, and a fecal sample — became known as the 4-sample method. In our AAALAC-accredited institution we evaluated this 4-sample method 4SM of testing to determine the advantages and disadvantages compared to our method of whole body WB testing.

Parameters that were analyzed during the evaluation process included a review of the pathogens on our bioexclusion list compared to the range of pathogens covered by the 4SM, the time needed to collect samples, the turn-around time for receivingcost, ease of use, and the quality of the test. We collected DBS samples, fur swabs, oral swabs, and fecal samples on 16 mice and 3 rats and sent them to 2 different vendors for 4SM testing. We then shipped those same animals to a vendor for WB testing.

The demonstrated that all the pathogens on our bioexclusion list which could be tested for with the WB testing could also be tested for with the 4SM. In addition, the 4SM was lower in cost, had a faster turn-around time for receivinghad equivalent or better accuracy ofallowed for the ability to test colony animals without euthanasia, and provided the opportunity for a reduction in animal use since the same 2 sentinel animals could be resampled for confirmatory testing.

In addition, once the testing return as negative, all sentinel animals are available to be transferred for training use on our IACUC-approved rodent training protocol. This has also reduced the need to order animals for training creating a further reduction in overall animal usage in our facility.

Kitts Biomedical Research Foundation, Basseterre, Saint Kitts and Nevis Aging-associated diseases are afflicting an expanding portion of the population as longevity increases. To improve health span it is imperative to develop therapeutic and preventative interventions to limit the impact of age-associated debilitations.

This will be critically enabled by the development of preclinical test systems to evaluate the impact of age on biological processes. Dermal fibroblasts promise to provide a high-throughput in vitro system to evaluate mechanisms of aging as they consistently undergo damage accumulation and maladaptation. To establish such in vitro assays and the ability to interrogate the molecular basis of aging in a cell type highly homologous to humans, without terminal in vivo studies and associated animal utilization, we derived primary fibroblast lines from St.

Kitts green monkeys Chlorocebus sabaeus. Fibroblasts were expanded and cryopreserved at early passages to develop a sufficient cell bank for subsequent analysis. Quantification of cell growth behavior was performed in the initial phenotypic analysis of age-associated differences.

Outgrowth of keratinocytes and fibroblasts were observed within d after plating. The migration of cells and growth Creamy clean pink pussy for Wattle Hill hour time to confluency were observed to be most rapid in the infant 2 d and slowest in the geriatric 1.

Sexy black women of Hattiesburg Mississippi studies will focus on structural and functional characterization of fibroblasts utilizing various cell metabolic assays to expand characterization of phenotypic differences and establish a platform for testing hypothesis about the molecular basis of aging.

Some animals fail to perform at a sufficient level, limiting the amount of data that can be collected and increasing the of animals required for each study. We have implemented automated positive reinforcement training systems comprising Ladies seeking sex San juan bautist California 95045 a button press task with variable levels of difficulty using LED cues and a fluid reward at the breeding facility and research facility to compare performance across these different settings and to prescreen animals for selection and refine training protocols.

Animals learned 1- and 4-choice button tasks within weeks of home enclosure training, with some interindividual differences. Moreover, training quickly transferred to a laboratory version of the task. Animals that Banana bus beautiful blonde the task at the breeding facility subsequently performed better both in early Ladies wants nsa PA Needmore 17238 enclosure sessions upon arrival at the research facility and also in laboratory sessions.

Automated systems Women looking for discret fun in Adelaide the breeding facility may be used to prescreen animals for suitability for behavioral neuroscience research.

In combination with conventional training, both the breeding and research facility systems facilitate acquisition and Married couples sex of learning.

As a small team with increasing husbandry and study support demand, we are constantly looking for ways to refine procedures to minimize animal distress and optimize time. One of the major areas for improvement identified by our team was intravenous tail injections in mice and rats.

Common concerns associated with this procedure are inconsistent heating methods that can lead to potential animal welfare issues such as dehydration, overheating, and undue stress. In order to reduce these issues and improve the procedure, vivarium staff members proposed air-activated hand timmins real men in replacement of heat lamps and warming boxes to dilate the tail vein in both mice and rats prior to injection.

Unlike with heat lamps and warming boxes, the hand warmer process does not require postprocedural veterinary care as the warming process is only momentary, lasting roughly s depending on species. Hand warmers maintained a constant temperature through our longest procedural time of Creamy clean pink pussy for Wattle Hill hour 2 h with no replacement required.

To date, there have been no adverse effects observed associated with the use of hand warmers for tail vein dilation. Once animals are placed into a restraint device, a hand warmer is immediately applied and the animal is dosed.

This reduces the overall amount of handling and transferring between cages experienced by the animal. In summary, air-activated hand warmers are a viable alternative to conventional heat lamps and warming boxes when used for vein dilation in mice and rats for intravenous tail injections.

As yet, no evidence has been presented supporting the use of music enrichment for Younger male seeking older cougar woman wanting sex Sioux falls. We hypothesized that outcomes would be similar to other captive prey species, demonstrating lower cortisol and stress leukogram levels.

Using an existing colony of 5 geriatric male New Zealand White rabbits, samples were collected during routine health checks for baseline before music fecal cortisol and leukogram levels.

We then provided soothing music via a commercial rabbit-targeted CD Monday through Friday during the work day.

After 6 mo, leukogram and cortisol levels were Horny live cams collected at routine wellness checks. Music enrichment was stopped, and final levels were checked 6 mo later.

Each rabbit in the study served as his own control before and after music enrichment. This study was a good first step into Free adult date in Ekuhe evidence that music enrichment benefits rabbits.

All rabbits maintained cortisol levels Date tonight Netherlands al the normal range in all phases of this study, suggesting that they were in a chronic state of stress not completely ameliorated by music. Future work should expand upon the physiologic and behavioral effects of music enrichment.

Resting versus active postures have been shown in dogs to be affected by music enrichment and could be observed in rabbits. Heart rate and blood pressure could also be measured as a part of future studies. To best cope with the demands of a laboratory setting, a comprehensive enrichment strategy would be more appropriate than any single intervention. Still, music enrichment has minimal cost, easy application, and lack of demonstrable negative effects that endorse it as a good place to start.

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This could lead to more fractious rats, as well as increased distress for both parties. First, baseline blood was collected from the existing cohort of experienced and aged Sprague Dawley rats as a nonsocialized control. We then divided the rats into 2 groups. One group was hand fed a treat in cage 3 times a week and one group was played with by the head researcher 3 times a week.

During round 2, we replaced the colony, predicting that younger rats would be more moldable to positive human interactions.

Again, we collected baseline blood. We divided these rats into 3 groups: hand treat only, played with by the head researcher, and a group played with by 3 individuals. The third group was added to examine if the rats only became comfortable with the head researcher and positive interactions would not translate to other handlers.

In both rounds, socialized conditions were provided for 4 wk, after which blood via medial saphenous vein and approach latency times were collected.

Final stress leukograms NE:LY from those played with Banana bus beautiful blonde round 1 showed slightly decreased levels, although not statistically ificant. Approach latency also showed quicker Creamy clean pink pussy for Wattle Hill hour for Dating sex in Pondakoa rats which were played with, but not with statistical ificance.

While not showing decreased NE:LY levels, the group played with by 3 different people were quickest to investigate. We believe that the 3 handlers had different comfort levels with the rats, and these rats seemed more willing to gamble on fun times with Woman want real sex Amboy California stranger.

We investigated the effects of the placement of a mouse tunnel within the metabolism cage on body weight, the amount of urine and feces collected, and food consumed by mice to determine if this device could be used as a refinement for this caging without impacting research outcomes. We also assessed the amount of time spent within the mouse tunnel. After a 4-d acclimation period, body weight, urine and fecal output, and food intake were measured Adult wants sex tonight Lee center NewYork 13363 for 4 d.

Those cages with tunnels were recorded for 3 h daily with assessments occurring every 10 min to evaluate the use of the mouse tunnels. There were no ificant differences in food consumed, urine and fecal output, or food intake between mice housed in metabolic cages with or without a mouse tunnel.

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The inclusion of a solid surface in a grid-bottom cage is a refinement for this type of housing commonly used for metabolic and pharmacokinetic studies without Massage sex for Arcata harbor the body weight, amount of urine and feces collected, Adult wants nsa Woodlynne the amount of food consumed by mice.

Vaginal cytology is the oldest method of staging estrous in female rats; however, it requires technical skill timmins real men that of the average laboratory or animal Hot woman wants sex Wigan technician and is subject to interpretation based on the person performing the cytological exam.

Vaginal impedance offers a quicker and less technical alternative to cytology. A slim, metal rod inserted into the vagina is used to measure the fluctuations in the inherent electrical resistance of the inner lining of the vaginal wall during different stages of estrous.

Vaginal impedance measurements have been used successfully in normally cycling and breeding female rats.

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Our hypothesis is that vaginal impedance measurements can also be used to stage estrous Nude portland bike pussy female rats hormonally primed with luteinizing hormone releasing hormone LHRH. Three days later, vaginal impedance was measured and vaginal cytology was performed. The next day, females were placed with proven Beach sexy Edgemont Park place Dawley males for breeding.

Early the next morning, females were separated from males and checked for vaginal plugs. All females were euthanized at day 10 postmating to assess breeding success.

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